用于2D凝胶的BioBasic/萃取缓冲液，含稀释剂、50ml尿素、硫脲、CHAPS和ASB-16/50ml/PL040
市场价：
¥1732.60
美元价：
866.30
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Product Description:Extraction Buffer for 2D Gel with Diluent, 50ml Urea, Thiourea, CHAPS and ASB-16: 2D buffers must solubilize proteins effectively without disturbing the native charge on the proteins. Urea, a common chaotrope, is widely used for solubilization and denaturation of proteins. One of the disadvantages of using urea is carbamylation. Urea in water exists in equilibrium with ammonium cyanate, of whose level increases with increasing temperature and pH. Cyanate reacts with α-amino and ε-amino groups of proteins and induces a change in the isoelectric point. This leads to artifacts in results and therefore carbamylation must be avoided. One way to minimize the risk of carbamylation is to prepare the urea based reagents fresh before each use. Bio Basic Inc. has developed a series of dry urea based pre-mixed and ready-to-use solubilization buffers. Simply add an appropriate volume of the supplied diluent buffer to the dry powder and then use directly to solubilize proteins, saving time and improving the quality of IEF/2D gel electrophoresis. 2D Sample Extraction Buffers are also designed to be used as rehydration buffers for IPG strips. 2D Sample Extraction Buffers are experimentally optimized with proprietary technologies and critical buffering and stabilizing agents, including urea, thiourea, Nonidet® P-40, CHAPS, and sulfobetaines. Our 2D Sample Extraction Buffers are designed to produce optimal protein extraction and significantly improved spot resolution for 2D gel analysis. A range of 2D Sample Extraction Buffers have been developed and depending on the nature of the samples, one or more of the buffers suitable for your applications can be ordered. PL037 is suitable for most applications, however for stronger solubilization effects, we recommend PL038-PL042.
Total Product Size:50ml
Individual Container Size:50ml
Number of Containers:1
Refrigeration Requirements:Freezer
Shipping Conditions:ICE
UNSPSC Code:41105323
UNSPSC Category:Electrophoresis Buffers
品牌介绍
Bio Basic Inc.是一家生命科学研究支持公司，成立于加拿大多伦多-总部位于安大略省万锦市和纽约阿默斯特。在Bio Basic，我们相信实验室服务和消耗品可以而且应该负担得起。我们直接制造基因，寡核苷酸，提取试剂盒，PCR试剂和许多其他研究必需品。我们的制造和供应链网络使我们能够提供业内最实惠的价格，而不会影响质量。科学研究的进步涉及许多阶段的漫长而艰辛的旅程。我们认为，通过支持科学研究的初始阶段（也称为“基础研究”），可以为整个过程的成功奠定重要基础。当科学产品和服务的成本上涨时，基础科学研究遭受的损失最大，这就是为什么我们的使命是促进基础科学研究。现在，支持基础研究将使将来的应用研究成功。
