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当前位置: 首页 > 产品中心 > Other_natural_proteins > BioBasic/10-250kDa宽量程蓝红色双色蛋白质梯状体,预染色,100ul/100ul/BZ0010R
商品详细BioBasic/10-250kDa宽量程蓝红色双色蛋白质梯状体,预染色,100ul/100ul/BZ0010R
BioBasic/10-250kDa宽量程蓝红色双色蛋白质梯状体,预染色,100ul/100ul/BZ0010R
BioBasic/10-250kDa宽量程蓝红色双色蛋白质梯状体,预染色,100ul/100ul/BZ0010R
商品编号: BZ0010R
品牌: BioBasic
市场价: ¥798.00
美元价: 399.00
产地: 美国(厂家直采)
公司:
产品分类: 其它天然蛋白
公司分类: Other_natural_proteins
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Product Description:Product Features - Prestained Protein LadderBroad range (10-250 kD)Dual-Color or Tri-ColorHigh size precision and lot consistencyClean and sharp bandsHigh membrane binding affinityHigh storage stabilityBio Basic prestained protein ladder introductionBio Basic prestained protein ladder is designed for estimating the approximate size of separated proteins on SDS-PAGE gel. The ladders are a mixture of recombinant proteins ranging from 10 kD to 250 kD. Red or green bands at 70 kD and 25 kD provide easy references for molecular weight identification. The molecular weights of the prestained ladders are confirmed using the Tris-Glycine SDS-PAGE system with an accuracy of >95% by using unstained protein ladders. The protein ladders are highly stable with minimal band broadening during storage. Products are conveniently packaged and ready to use, with no heating, diluting or additional reducing agent required.Bio Basic Prestained protein ladder has high size precisionTo perform molecular weight (MW) size precision evaluation, both unstained and prestained ladders were resolved on a 4-15% gradient SDS-PAGE Gel (BioRad #4561083) followed by Coomassie BB staining (left panel). MWs of all protein bands were indicated at their migration positions (right panel). As seen in lane 3, 4 and 5, some prestained protein bands show significant MWs inconsistencies between BioRad’s and Thermo’s products. Similar inconsistent bands are also observed between two ladders from the same manufacturer (Thermo #26616 and #26619). Generally, unstained protein ladders are considered to be more precise. Here, two unstained ladders (Thermo #26630 and NEB #7703, a third-party’s product) were included as standard references (lane 1&2). When compared with unstained ladders, there are obvious imprecise prestained bands in both popular brands’ products. Interestingly, the MWs of Thermo’s prestained ladders (#26616 and #26619) do not match their own unstained protein ladder (#26630). There are no significant imprecise bands in the Bio Basic ladder (lane 6). It should be noted that presteined ladders were originally designed for approximative MW reference and therefore, neither represent an absolute standard.*Normally, all prestained protein ladders have lot-to-lot variation. This evaluation only represented the tested lot and did not intend to conclude a judgement of all other lots of listed brands’ products.**The evaluation was performed on Tris-Glycine buffer system. The mobility of prestained proteins can vary in different SDS-PAGE buffer systems.
Protein
Bio Basic Prestained protein ladder has high stabilityWe fully understand that storage stability is very important for bulk purchasers. Bio Basic prestained protein ladders have excellent stability and can be stored at frozen for long term. For storage stability test, BZ0010R was stored at -20°C and 37°C, respectively, for one month and resolved on 12.5% Laemmli SDS-PAGE gel. Compared with storage in -20°C, BZ0010R only slightly faded (lane 1&2) and little protein degradation occurred (lane 3&4) after one-month storage at 37°C. This result clearly showed that Bio Basic prestained ladder is highly stable even in such harsh condition.
Shipment and storageProducts are shipped on ice pack and should be store frozen upon receipt. Occasional thawing partly during shipping will not affect the quality and performance because our protein ladders are substantial stable in ambient temperature. We recommend to store ladders at -80°C for long term storage. When you subpackage, product should be equilibrated at 4°C overnight and further let it stay at room temperature until completely thawed. Mix thoroughly to make sure no insoluble precipitate occurs. If necessary, warm it at 37°C to dissolve any precipitate (no heat!). Aliquoting recommended. Store all aliquots as above or at -20°C for one year.11 bands: 10, 15, 20, 25 (Red), 35, 40, 50, 70 (Red), 100, 150, 250 kDa
Total Product Size:100ul
Individual Container Size:100ul
Number of Containers:1
Refrigeration Requirements:Freezer
Shipping Conditions:ICE
UNSPSC Code:41105337
UNSPSC Category:Protein Molecular Weight Markers
品牌介绍
Bio Basic Inc.是一家生命科学研究支持公司,成立于加拿大多伦多-总部位于安大略省万锦市和纽约阿默斯特。在Bio Basic,我们相信实验室服务和消耗品可以而且应该负担得起。我们直接制造基因,寡核苷酸,提取试剂盒,PCR试剂和许多其他研究必需品。我们的制造和供应链网络使我们能够提供业内最实惠的价格,而不会影响质量。科学研究的进步涉及许多阶段的漫长而艰辛的旅程。我们认为,通过支持科学研究的初始阶段(也称为“基础研究”),可以为整个过程的成功奠定重要基础。当科学产品和服务的成本上涨时,基础科学研究遭受的损失最大,这就是为什么我们的使命是促进基础科学研究。现在,支持基础研究将使将来的应用研究成功。