BioBasic/无缝克隆主混合物(套件)/40RXN/B632219-0040
商品编号:
B632219-0040
品牌:
BioBasic
市场价:
¥6125.40
美元价:
3062.70
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Product Description:Seamless cloning Master Mix (Kits): The Seamless Cloning Master Mix is a ready-to-use reaction mix for seamless cloning reactions. It allows for successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility. It has been rapidly adopted by the synthetic biology community due to its ease-of-use, flexibility and suitability for large DNA constructs. The Seamless Cloning Master Mix efficiently joins multiple overlapping DNA fragments in a single-tube isothermal reaction. The system allows the cloning of the DNA fragments into virtually any linearized Escherichia. coli vector, requires no preexisting recombination sites or extra DNA sequences, and eliminates the need for extensive enzymatic treatments of the DNA such as restriction and ligation. The enzyme mix provided with the Seamless Cloning Master Mix recognizes and precisely assembles the DNA fragments sharing a 15-20base pair (bp) end homology that can be created by PCR-amplification. The end result is a double-stranded fully sealed DNA molecule that can serve as template for PCR, RCA or a variety of other molecular biology applications, including direct transformation. Using the Seamless Cloning Master Mix, you can clone multiple DNA fragments into a single vector without subcloning, create modular expression vectors with interchangeable parts, construct seamless fusion proteins, delete and replace DNA segments, make internal protein fusions, swap tags on a gene, add UTRs to a cDNA, insert restriction sites, and more.
Number of Containers:1
Shipping Conditions:RT
UNSPSC Code:41116133
UNSPSC Category:DNA Ligation Kits
品牌介绍
Bio Basic Inc.是一家生命科学研究支持公司,成立于加拿大多伦多-总部位于安大略省万锦市和纽约阿默斯特。在Bio Basic,我们相信实验室服务和消耗品可以而且应该负担得起。我们直接制造基因,寡核苷酸,提取试剂盒,PCR试剂和许多其他研究必需品。我们的制造和供应链网络使我们能够提供业内最实惠的价格,而不会影响质量。科学研究的进步涉及许多阶段的漫长而艰辛的旅程。我们认为,通过支持科学研究的初始阶段(也称为“基础研究”),可以为整个过程的成功奠定重要基础。当科学产品和服务的成本上涨时,基础科学研究遭受的损失最大,这就是为什么我们的使命是促进基础科学研究。现在,支持基础研究将使将来的应用研究成功。