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商品详细BioBasic/无干扰蛋白质浓度测定试剂盒/250preps/SK3071
BioBasic/无干扰蛋白质浓度测定试剂盒/250preps/SK3071
BioBasic/无干扰蛋白质浓度测定试剂盒/250preps/SK3071
商品编号: SK3071
品牌: BioBasic
市场价: ¥1570.80
美元价: 785.40
产地: 美国(厂家直采)
公司:
产品分类: 聚合物
公司分类: juhewu
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Product Description:Non-Inerfering Protien Concentration Determination Kit: This kit provides a highly sensitive, colorimetric protein assay that overcomes interference of common laboratory agents present in protein solutions, such as detergents (non-ionic, ionic and zwitterionic), reducing agents (β-mercaptoethanol, DTT), chelating agents (EDTA), amines (Tris), sugars, and strong chaotropic buffers. The kit is suitable for protein concentration determination during protein purification, electrophoresis, and many other protein biology applications. A variety of protein samples can be used from cellular fractions, tissue, 2D lysates to chromatography fractions. In this assay, Precipitation Reagent is added to the protein solution to rapidly precipitate total protein. Interfering agents in the supernatant are discarded. Protein concentration is then determined by mixing the protein with an alkaline solution containing a known concentration of copper salt. The copper ions bind to the peptide backbone and the unbound copper is measured with a colorproducing agent. As the concentration of protein increases, the concentration of unbound copper decreases, thus the color density is inversely proportional to the amount of protein present in solution. This assay is not affected by protein side chains or groups, which minimizes protein-toprotein variation. This fact, when combined with the strategy of removing interfering agents from the protein solution, allows for a highly reliable determination of protein concentration.
Total Product Size:250preps
Individual Container Size:250preps
Number of Containers:1
Refrigeration Requirements:Freezer
Shipping Conditions:ICE
UNSPSC Code:41116012
UNSPSC Category:Protein Assays
品牌介绍
Bio Basic Inc.是一家生命科学研究支持公司,成立于加拿大多伦多-总部位于安大略省万锦市和纽约阿默斯特。在Bio Basic,我们相信实验室服务和消耗品可以而且应该负担得起。我们直接制造基因,寡核苷酸,提取试剂盒,PCR试剂和许多其他研究必需品。我们的制造和供应链网络使我们能够提供业内最实惠的价格,而不会影响质量。科学研究的进步涉及许多阶段的漫长而艰辛的旅程。我们认为,通过支持科学研究的初始阶段(也称为“基础研究”),可以为整个过程的成功奠定重要基础。当科学产品和服务的成本上涨时,基础科学研究遭受的损失最大,这就是为什么我们的使命是促进基础科学研究。现在,支持基础研究将使将来的应用研究成功。