BioBasic/dNTP混合物（10mM）/0.5ml/DD0056
市场价：
¥370.80
美元价：
185.40
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Product Description:dNTP mixture (10mM)Product Summary
DNase, RNase: None detected.Suitable for use in the Polymerase Chain Reaction (PCR).PCR SuitabilitydNTP Mix is a solution containing each of the four deoxynucleotides as follows: 10 mM dATP10 mM dCTP10 mM dGTP10 mM dTTPdNTP Mix was tested at a final concentration of 200 μM in a reaction mixture containing 10 mM Tris-HCl, pH 8.3 at 25°C, 50 mM KCl, 1.5 mM MgCl2, 0.001% (w/v) gelatin, primers defining an approximately 500 base pair region of λ DNA at 1.0 μM each, λ DNA template at 1 ng/100 μl, and Taq DNA polymerase at 2.5 units/100 μl. The reaction underwent 25 cycles of 94 °C to denature the double stranded DNA, 55°C to anneal the DNA segments, and 72°C to extend the DNA segments. A single band of approximately 500 base pairs was visualized following electrophoresis of the reaction product in a 1.5% agarose gel.Endonuclease-ExonucleaseOne μg of λ Hind III fragments was incubated for 16 hours at 37°C with dNTP Mix at a final concentration of 5 mM in a 50 μl reaction mixture containing 30 mM Tris- HCl, pH 7.8, 50 mM NaCl and 10 mM MgCl2. No degradation of the DNA fragments was detected following agarose gel electrophoresis. Detection limit: Degradation of 10% of the DNA substrate is detectable.Endonuclease (Nickase)One μg of pBR322 DNA was incubated for 16 hours at 37°C with dNTP Mix at a final concentration of 5 mM in a 50 μl reaction mixture containing 30 mM Tris-HCl, pH 7.8, 50 mM NaCl and 10 mM MgCl2. No conversion of the covalently closed circular DNA to the nicked or linear form was observed following agarose gel electrophoresis. Detection limit: Conversion of 1% of the DNA substrate is detectable.RNaseTwo μg of transfer RNA were incubated for 16 hours at 37°C with dNTP Mix at a final concentration of 5 mM in a 50 μl reaction mixture containing 30 mM Tris-HCl, pH 7.8, 50 mM NaCl and 10 mM MgCl2. No degradation of the tRNA was detected following polyacrylamide gel electrophoresis. Detection limit: Degradation of 10% of the tRNA substrate is detectable.
DNase, RNase: None detected.Suitable for use in the Polymerase Chain Reaction (PCR).PCR SuitabilitydNTP Mix is a solution containing each of the four deoxynucleotides as follows: 10 mM dATP10 mM dCTP10 mM dGTP10 mM dTTPdNTP Mix was tested at a final concentration of 200 μM in a reaction mixture containing 10 mM Tris-HCl, pH 8.3 at 25°C, 50 mM KCl, 1.5 mM MgCl2, 0.001% (w/v) gelatin, primers defining an approximately 500 base pair region of λ DNA at 1.0 μM each, λ DNA template at 1 ng/100 μl, and Taq DNA polymerase at 2.5 units/100 μl. The reaction underwent 25 cycles of 94 °C to denature the double stranded DNA, 55°C to anneal the DNA segments, and 72°C to extend the DNA segments. A single band of approximately 500 base pairs was visualized following electrophoresis of the reaction product in a 1.5% agarose gel.Endonuclease-ExonucleaseOne μg of λ Hind III fragments was incubated for 16 hours at 37°C with dNTP Mix at a final concentration of 5 mM in a 50 μl reaction mixture containing 30 mM Tris- HCl, pH 7.8, 50 mM NaCl and 10 mM MgCl2. No degradation of the DNA fragments was detected following agarose gel electrophoresis. Detection limit: Degradation of 10% of the DNA substrate is detectable.Endonuclease (Nickase)One μg of pBR322 DNA was incubated for 16 hours at 37°C with dNTP Mix at a final concentration of 5 mM in a 50 μl reaction mixture containing 30 mM Tris-HCl, pH 7.8, 50 mM NaCl and 10 mM MgCl2. No conversion of the covalently closed circular DNA to the nicked or linear form was observed following agarose gel electrophoresis. Detection limit: Conversion of 1% of the DNA substrate is detectable.RNaseTwo μg of transfer RNA were incubated for 16 hours at 37°C with dNTP Mix at a final concentration of 5 mM in a 50 μl reaction mixture containing 30 mM Tris-HCl, pH 7.8, 50 mM NaCl and 10 mM MgCl2. No degradation of the tRNA was detected following polyacrylamide gel electrophoresis. Detection limit: Degradation of 10% of the tRNA substrate is detectable.Total Product Size:0.5ml
Individual Container Size:0.5ml
Number of Containers:1
Refrigeration Requirements:Freezer
Shipping Conditions:ICE
UNSPSC Code:12352208
UNSPSC Category:Nucleic Acid Bases
品牌介绍
Bio Basic Inc.是一家生命科学研究支持公司，成立于加拿大多伦多-总部位于安大略省万锦市和纽约阿默斯特。在Bio Basic，我们相信实验室服务和消耗品可以而且应该负担得起。我们直接制造基因，寡核苷酸，提取试剂盒，PCR试剂和许多其他研究必需品。我们的制造和供应链网络使我们能够提供业内最实惠的价格，而不会影响质量。科学研究的进步涉及许多阶段的漫长而艰辛的旅程。我们认为，通过支持科学研究的初始阶段（也称为“基础研究”），可以为整个过程的成功奠定重要基础。当科学产品和服务的成本上涨时，基础科学研究遭受的损失最大，这就是为什么我们的使命是促进基础科学研究。现在，支持基础研究将使将来的应用研究成功。
