BioBasic/9K系列高Taq DNA聚合酶，含10mM dNTP混合物/5000U/9K-001-0005/9K-001-0006/9K-001-0020
品牌：
BioBasic
市场价：
¥44280.00
美元价：
22140.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Product Description:High-Taq DNA Polymerase with 10mM dNTP Mix (5000U)
High-Taq DNA Polymerase (2.5U/ I)OX High-Taq reaction Buffer10 mM dNTP Mix (each 1OmM}Band SharpenerA. Template (temperature cycle)- Animal genomic DNA50-200 ng (30-45 cycles) 10-50 ng (35-50 cycles)- Bacterial genomic DNA10-50 ng (30-40 cycles) 1-5 ng (35-50 cycles)- Plasmid and lamda DNA1-5 ng (35-40 cycles)B. High-Taq DNA Polymerase1.25unit (0.5µI) per 50µI reaction is recommended when amplifying animal genomic DNA. Please use 2.5-5 unit in case target size is >3kb.In case the target is >3kb, we recommend EF-.TaqC. Band Sharpener Band Sharpener Is not necessary for regular PCR conditions. In case fragment include high GC region or hard to amplifycomplex secondary structures, please add Band Sharpener to a final concentration of 0.5x-2x (5-20µI for 50µI reaction) to reaction mixture, as optimization is required (see protocol below).D. Primer design- Primer can be designed using a primer design software or manually.- Avoid repeated sequence at 3" end.- In case 3"-end is G+C rich, the end have to be A or T.- In case 3" end is A+T rich, the end have to be G or C.- It is recommended that Tm of the designed primers is >64C and AT >58C.E. Extension time- Normally, extension time is 1 min/kb. In case target is >3kb, the extension time should be increased to 1.5-2.0 min/kb.
High-Taq DNA Polymerase (2.5U/ I)OX High-Taq reaction Buffer10 mM dNTP Mix (each 1OmM}Band SharpenerA. Template (temperature cycle)- Animal genomic DNA50-200 ng (30-45 cycles) 10-50 ng (35-50 cycles)- Bacterial genomic DNA10-50 ng (30-40 cycles) 1-5 ng (35-50 cycles)- Plasmid and lamda DNA1-5 ng (35-40 cycles)B. High-Taq DNA Polymerase1.25unit (0.5µI) per 50µI reaction is recommended when amplifying animal genomic DNA. Please use 2.5-5 unit in case target size is >3kb.In case the target is >3kb, we recommend EF-.TaqC. Band Sharpener Band Sharpener Is not necessary for regular PCR conditions. In case fragment include high GC region or hard to amplifycomplex secondary structures, please add Band Sharpener to a final concentration of 0.5x-2x (5-20µI for 50µI reaction) to reaction mixture, as optimization is required (see protocol below).D. Primer design- Primer can be designed using a primer design software or manually.- Avoid repeated sequence at 3" end.- In case 3"-end is G+C rich, the end have to be A or T.- In case 3" end is A+T rich, the end have to be G or C.- It is recommended that Tm of the designed primers is >64C and AT >58C.E. Extension time- Normally, extension time is 1 min/kb. In case target is >3kb, the extension time should be increased to 1.5-2.0 min/kb.Total Product Size:9K-001-0005 (250U)9K-001-0006 (4x250U)9K-001-0020 (5000U)
Individual Container Size:1
Number of Containers:1
Refrigeration Requirements:Freezer
Shipping Conditions:ICE
UNSPSC Code:41106300
UNSPSC Category:PCR-related Kits
品牌介绍
Bio Basic Inc.是一家生命科学研究支持公司，成立于加拿大多伦多-总部位于安大略省万锦市和纽约阿默斯特。在Bio Basic，我们相信实验室服务和消耗品可以而且应该负担得起。我们直接制造基因，寡核苷酸，提取试剂盒，PCR试剂和许多其他研究必需品。我们的制造和供应链网络使我们能够提供业内最实惠的价格，而不会影响质量。科学研究的进步涉及许多阶段的漫长而艰辛的旅程。我们认为，通过支持科学研究的初始阶段（也称为“基础研究”），可以为整个过程的成功奠定重要基础。当科学产品和服务的成本上涨时，基础科学研究遭受的损失最大，这就是为什么我们的使命是促进基础科学研究。现在，支持基础研究将使将来的应用研究成功。
